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a. Full amino acid sequence of the crystallized protein. H. pylori TlpD from strain J99 was expressed with an N-terminal 6x His-tag containing a TEV cleavage site; an N-terminal Ser remains following TEV cleavage. Zinc-binding residues are highlighted in pink. Shown bottom are purification steps <t>from</t> <t>Ni-NTA</t> purification and TEV-cleavage for the protein used for crystallography. b. Shown is a representative image of the typical small clusters of poorly-diffracting crystals that TlpD readily forms in a variety of PEG and ammonium sulfate-based crystallization conditions. c. An image of the drop in which large and well-diffracting TlpD crystals grew that yielded the datasets in this study. d. An image of the P 1 crystal (harvested from c) that yielded the initial solution. The crystal was flash frozen and x-ray diffraction data collected under cryo conditions. e. A representative diffraction image from the crystal in d.
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a. Full amino acid sequence of the crystallized protein. H. pylori TlpD from strain J99 was expressed with an N-terminal 6x His-tag containing a TEV cleavage site; an N-terminal Ser remains following TEV cleavage. Zinc-binding residues are highlighted in pink. Shown bottom are purification steps <t>from</t> <t>Ni-NTA</t> purification and TEV-cleavage for the protein used for crystallography. b. Shown is a representative image of the typical small clusters of poorly-diffracting crystals that TlpD readily forms in a variety of PEG and ammonium sulfate-based crystallization conditions. c. An image of the drop in which large and well-diffracting TlpD crystals grew that yielded the datasets in this study. d. An image of the P 1 crystal (harvested from c) that yielded the initial solution. The crystal was flash frozen and x-ray diffraction data collected under cryo conditions. e. A representative diffraction image from the crystal in d.
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Average 94 stars, based on 1 article reviews
his60 ni gravity column - by Bioz Stars, 2026-04
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a. Full amino acid sequence of the crystallized protein. H. pylori TlpD from strain J99 was expressed with an N-terminal 6x His-tag containing a TEV cleavage site; an N-terminal Ser remains following TEV cleavage. Zinc-binding residues are highlighted in pink. Shown bottom are purification steps <t>from</t> <t>Ni-NTA</t> purification and TEV-cleavage for the protein used for crystallography. b. Shown is a representative image of the typical small clusters of poorly-diffracting crystals that TlpD readily forms in a variety of PEG and ammonium sulfate-based crystallization conditions. c. An image of the drop in which large and well-diffracting TlpD crystals grew that yielded the datasets in this study. d. An image of the P 1 crystal (harvested from c) that yielded the initial solution. The crystal was flash frozen and x-ray diffraction data collected under cryo conditions. e. A representative diffraction image from the crystal in d.
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a. Full amino acid sequence of the crystallized protein. H. pylori TlpD from strain J99 was expressed with an N-terminal 6x His-tag containing a TEV cleavage site; an N-terminal Ser remains following TEV cleavage. Zinc-binding residues are highlighted in pink. Shown bottom are purification steps from Ni-NTA purification and TEV-cleavage for the protein used for crystallography. b. Shown is a representative image of the typical small clusters of poorly-diffracting crystals that TlpD readily forms in a variety of PEG and ammonium sulfate-based crystallization conditions. c. An image of the drop in which large and well-diffracting TlpD crystals grew that yielded the datasets in this study. d. An image of the P 1 crystal (harvested from c) that yielded the initial solution. The crystal was flash frozen and x-ray diffraction data collected under cryo conditions. e. A representative diffraction image from the crystal in d.

Journal: bioRxiv

Article Title: Structure and signaling mechanism of Helicobacter pylori transducer-like protein D

doi: 10.64898/2026.01.16.699579

Figure Lengend Snippet: a. Full amino acid sequence of the crystallized protein. H. pylori TlpD from strain J99 was expressed with an N-terminal 6x His-tag containing a TEV cleavage site; an N-terminal Ser remains following TEV cleavage. Zinc-binding residues are highlighted in pink. Shown bottom are purification steps from Ni-NTA purification and TEV-cleavage for the protein used for crystallography. b. Shown is a representative image of the typical small clusters of poorly-diffracting crystals that TlpD readily forms in a variety of PEG and ammonium sulfate-based crystallization conditions. c. An image of the drop in which large and well-diffracting TlpD crystals grew that yielded the datasets in this study. d. An image of the P 1 crystal (harvested from c) that yielded the initial solution. The crystal was flash frozen and x-ray diffraction data collected under cryo conditions. e. A representative diffraction image from the crystal in d.

Article Snippet: The clarified lysate was loaded onto a gravity-flow Ni-NTA agarose column (Qiagen) pre-equilibrated with lysis buffer and incubated for 30–60 min, with the lysate passed over the resin twice to enhance binding.

Techniques: Sequencing, Binding Assay, Purification, Crystallization Assay